Spatial Remodeling of the Skeletal Muscle Proteome During Lipid-Induced Insulin Resistance

PhD defence by Júlia Prats Quesada

Assessment Committee

[Associate professor Marta Guasch Ferré, NNF Center for Basic Metabolic Research (CBMR), University of Copenhagen (Chairperson)
Associate professor Jean Farup, Aarhus University
Dr Daniel Fazakerley, University of Cambridge

Supervisors

Associate Professor Atul Shahaji Deshmukh
Clara Prats Gavalda
Postdoc Jeppe Kjærgaard Northcote

Department

Novo Nordisk Foundation Center for Basic Metabolic Research

Graduate Programme

Basic Metabolic Research

Place

Building: Floor 1, Building 9b, Room: Henrik Dam Auditorium, Blegdamsvej 3B, 2200 Copenhagen N

Email address to gain access to the thesis: julia.quesada@sund.ku.dk.
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Short description of the thesis

Skeletal muscle is the primary site of insulin-stimulated glucose uptake, and its metabolic dysfunction is a major contributor to type 2 diabetes. While chronic lipid overload is known to impair insulin action, the molecular mechanisms remain incompletely understood. Most research has focused on which proteins are present and how active they are, but where proteins are located inside the cell matters just as much. This thesis introduces a spatial proteomics workflow integrating subcellular fractionation, high-resolution mass spectrometry, and computational modelling to systematically map protein localization in muscle cells under lipid overload and insulin stimulation.